|
Genetic interval (chromosome)
|
No. testeda
|
No. recombinanta
|
Recombinant freq. (%)
|
cMb
|
cM in wild-typec
|
Fold reductionc
|
|---|
|
A. rec12-D15
|
|
lys1 – his1 (I)
|
500
|
3
|
0.6
|
0.6
|
89
|
148
|
|
lys1 – ade4 (I)
|
500
|
8
|
1.6
|
1.6
|
284
|
177
|
|
his1 – ade2 (I)
|
500
|
2
|
0.4
|
0.4
|
57
|
142
|
|
his1 – tps19 (I)
|
500
|
2
|
0.4
|
0.4
|
143
|
357
|
|
ade2 – tps19 (I)
|
500
|
0
|
≤ 0.2
|
≤ 0.2
|
86
|
≤ 430
|
|
his4 – ade1 (II)
|
500
|
6
|
1.2
|
1.2
|
63
|
53
|
|
ade6 – arg1 (III)
|
500
|
1
|
0.2
|
0.2
|
97
|
486
|
| | | | | |
mean =
|
256 ± 154
|
|
B.
rec12-Y98F
|
|
lys1 – his1 (I)
|
500
|
3
|
0.6
|
0.6
|
89
|
148
|
|
lys1 – ade4 (I)
|
500
|
17
|
3.4
|
3.5
|
284
|
81
|
|
his1 – ade2 (I)
|
500
|
1
|
0.2
|
0.2
|
57
|
285
|
|
his1 – tps19 (I)
|
500
|
1
|
0.2
|
0.2
|
143
|
715
|
|
ade2 – tps19 (I)
|
500
|
0
|
≤ 0.2
|
≤ 0.2
|
86
|
≤ 430
|
|
his4 – ade1 (II)
|
500
|
5
|
1.0
|
1.0
|
63
|
63
|
|
ade6 – arg1 (III)
|
500
|
4
|
0.8
|
0.8
|
97
|
121
|
| | | | | |
mean =
|
263 ± 220
|
- aSpore colonies were replica plated on non-selective and selective media; the number of colonies recombinant for each genetic interval are indicated. Diploid (potentially complementing) spore colonies were excluded from analysis. bGenetic distance calculated using the mapping function of Haldane [66]. cFold reduction in crossing over relative to published map distances in wild-type cells (NCBI Entrez Genome) [36]. The strains crossed were: WSP1826 × WSP1967; WSP1825 × WSP1962; WSP1967 × WSP1973; WSP1967 × WSP1973; WSP1967 × WSP1973; WSP2015 × WSP2018; WSP2026 × WSP2024; WSP2020 × WSP1966; WSP1670 × WSP1968; WSP1966 × WSP2027; WSP1966 × WSP2027; WSP1966 × WSP2027; WSP2014 × WSP2011; WSP1809 × WSP2028. Strain genotypes are provided in Table 3.
