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Table 1 Intergenic (crossover) recombination in rec12 mutants.

From: Distinct functions of S. pombeRec12 (Spo11) protein and Rec12-dependent crossover recombination (chiasmata) in meiosis I; and a requirement for Rec12 in meiosis II

Genetic interval (chromosome) No. testeda No. recombinanta Recombinant freq. (%) cMb cM in wild-typec Fold reductionc
A. rec12-D15
lys1 – his1 (I) 500 3 0.6 0.6 89 148
lys1 – ade4 (I) 500 8 1.6 1.6 284 177
his1 – ade2 (I) 500 2 0.4 0.4 57 142
his1 – tps19 (I) 500 2 0.4 0.4 143 357
ade2 – tps19 (I) 500 0 ≤ 0.2 ≤ 0.2 86 ≤ 430
his4 – ade1 (II) 500 6 1.2 1.2 63 53
ade6 – arg1 (III) 500 1 0.2 0.2 97 486
      mean = 256 ± 154
B. rec12-Y98F
lys1 – his1 (I) 500 3 0.6 0.6 89 148
lys1 – ade4 (I) 500 17 3.4 3.5 284 81
his1 – ade2 (I) 500 1 0.2 0.2 57 285
his1 – tps19 (I) 500 1 0.2 0.2 143 715
ade2 – tps19 (I) 500 0 ≤ 0.2 ≤ 0.2 86 ≤ 430
his4 – ade1 (II) 500 5 1.0 1.0 63 63
ade6 – arg1 (III) 500 4 0.8 0.8 97 121
      mean = 263 ± 220
  1. aSpore colonies were replica plated on non-selective and selective media; the number of colonies recombinant for each genetic interval are indicated. Diploid (potentially complementing) spore colonies were excluded from analysis. bGenetic distance calculated using the mapping function of Haldane [66]. cFold reduction in crossing over relative to published map distances in wild-type cells (NCBI Entrez Genome) [36]. The strains crossed were: WSP1826 × WSP1967; WSP1825 × WSP1962; WSP1967 × WSP1973; WSP1967 × WSP1973; WSP1967 × WSP1973; WSP2015 × WSP2018; WSP2026 × WSP2024; WSP2020 × WSP1966; WSP1670 × WSP1968; WSP1966 × WSP2027; WSP1966 × WSP2027; WSP1966 × WSP2027; WSP2014 × WSP2011; WSP1809 × WSP2028. Strain genotypes are provided in Table 3.