Figure 8
From: Calyculin A, an enhancer of myosin, speeds up anaphase chromosome movement
Backwards chromosome movements in crane-fly spermatocytes treated with CalA are not actin or myosin dependent. (A) Chromosome movements in a cell treated with CalA in anaphase (left arrow), and with BDM in CalA added after the chromosomes started to move backwards (right arrow). Backwards movements are not affected by the myosin inhibitor BDM. The dashed line represents linear regression through the circles with dots before addition of CalA and the solid line through the circles with dots after addition of CalA. (B) Chromosome movements in a cell treated with CalA in anaphase (left arrow), and with LatB in CalA added after the chromosomes reached the poles, at the time when they started to move backwards (right arrow). Backwards movements are not affected by actin inhibitor LatB. The dashed line represents linear regression through the circles with dots before addition of CalA and the solid line through the circles with dots after addition of CalA.