Calyculin A effects on anaphase chromosome movement and cytokinesis in crane-fly spermatocytes. (A) CalA added during anaphase (arrow) causes chromosomes to accelerate and to move backwards after they reached the poles (one half-bivalent pair is shown). Dashed line represents linear regression through the crosses before addition of CalA and the solid line after addition of CalA. (B) CalA added during metaphase (arrow), a few minutes before anaphase onset, causes chromosomes to move fast during subsequent anaphase and to move backwards after reaching the poles (one half-bivalent pair is shown). (C) CalA added during cytokinesis (downward arrow) stops furrow ingression and reverses cytokinesis. Cells do not resume cytokinesis after washing out CalA (upward arrow).