Table 1 Cytogenetic analysis of mouse metaphase II oocytes following in vitro exposure to the proteasome inhibitor MG-132
MG-132 (mcg/ml) | No. cells analyzed | No. MI oocytes (%) | No. MII oocytes (%) | No. premature centromere separation (%) | No. single chromatids (%) | No. haploid (%) | No. hypoploid (%) | No. hyperploid (%) |
|---|---|---|---|---|---|---|---|---|
Control | 130 | 13 (10.0)a | 117 (90.0) | 43 (36.8)a | 1 (0.9)a | 101 (86.3) | 16 (13.7) | -0-a |
5.0 | 158 | 19 (12.0)a | 139 (88.0) | 38 (27.3) | 3 (2.2)a,b | 121 (87.1) | 18 (12.9) | -0-a |
7.5 | 191 | 26 (13.6)a | 165 (86.4) | 44 (26.7) | 11 (6.7)b | 142 (86.1) | 18 (10.9) | 5 (3.0)b |
10.0 | 125 | 53 (42.4)b | 72 (57.6) | 13 (18.1)b | 11 (15.3)c | 62 (86.1) | 3 (4.2) | 7 (9.7)c |